High yield Plasmid Miniprep with AHN myTube® Spin Columns
Plasmid Miniprep with High-efficiency plasmid DNA binding
Protocols for commercially available plasmid miniprep kits typically involve some preparatory steps that separate plasmid DNA from chromosomal DNA and other cell components. The separated plasmid DNA in solution is then centrifuged through AHN myTube® spin columns with glass bead filters. The uniquely formulated glass bead filter technology is well optimized for high-efficiency (>99.99%) DNA binding to maximize plasmid preparation yields. The plasmid DNA remains bound to the glass bead filter during centrifugation while impurities are separated into the collection tubes with the flow-through.
Plasmid Preparation with Excellent DNA retention during washing
After binding plasmid DNA to the glass bead filters of AHN myTube® spin columns, most plasmid miniprep protocols recommend a wash step with ethanol-based solutions. Washing the glass bead filter membranes with ethanol removes any impurities that may persist after the binding cycle. AHN myTube® spin columns are fine-tuned to retain DNA throughout the wash cycles, reducing the risk of low-yield plasmid DNA purification. The filter design also ensures maximum ethanol removal during centrifugation so as to minimize alcohol interference in downstream processes of plasmid miniprep.
Plasmid Miniprep with Easy plasmid DNA elution
The reversible binding of plasmid DNA to the glass bead filters of AHN myTube® spin columns makes quick work of the elution step of plasmid miniprep. By adding nuclease-free water or a low-salt elution buffer to the spin column and centrifuging into a clean collection tube, the glass bead filters release all the bound plasmid DNA. All AHN myTube® spin columns with glass bead filters are optimized for reversible DNA binding to maximize plasmid recovery with each plasmid miniprep elution step.
Plasmid DNA Purification with Premium quality Spin Columns
AHN myTube® spin columns made from the best quality raw materials and they are well optimized for the best results in each step of plasmid preparation. Each AHN myTube® spin column is carefully crafted from premium-grade polypropylene that is optimized for minimal chemical interaction with plasmid miniprep reagents. This decreases the chances of accidental carryover of unwanted molecules. The glass bead filter technology offers dependable DNA binding that results in dependable plasmid miniprep yields.
Graduated collection tubes for Plasmid Miniprep
The AHN myTube® spin column system has graduated collection tubes that simplify the monitoring of flow-through volumes during centrifugation. By quickly noting the volume of solution collected in the flow-through, it is easy to notice pipetting errors without restarting the whole plasmid preparation process. Collection tubes are supplied in 1.5mL-2.0mL volume capacities that accommodate the standard AHN spin column size.
Try out AHN myTube® spin columns in your next plasmid miniprep
You can order free samples of AHN myTube® spin columns to test them out in your next plasmid DNA purification. Experience the innovative features of AHN myTube® spin columns and take your plasmid miniprep to the next level. With AHN you get more value for your money with each plasmid miniprep.
AHN Biotechnologie GmbH is a company located in Nordhausen – Germany, which is specialized in the development, manufacturing and distribution of LIQUID HANDLING PRODUCTS for the use in laboratories worldwide. The product range includes pipette tips, filter tips, pipettes, tubes and products for filtration and purification of biomolecules. For over 20 years AHN Biotechnologie GmbH has been an OEM manufacturer of consumables for many top life science brands and distribution companies in Germany and worldwide. Our state of the art production facility meets and exceeds the strict requirements of the most well-known brands, we are supplying for. Our know-how within pipette tips‘ production has now been strengthened by the implementation of newly developed Swiss molds, leading to the launch of new tips‘ lines, matching the strict standards of modern lab industry. Our goal is to offer the market’s best value for money on pipette tips and other AHN products. [url=https://ahn-bio.de/?utm_source=PresseBox&utm_medium=PresseBox&utm_campaign=PresseBox]Click here for more information[/url]
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Telefon: +49(0) 3631 65242-0
E-Mail: mb@cappahn.com