First Provider in Germany: CeGaT Offers Sequencing Services Based on PacBio’s SMRT Technology

The Tübingen-based company makes state-of-the-art sequencing technologies available to all life scientists. With the implementation of Full-Length 16S Sequencing into its portfolio, CeGaT now offers sequencing of the entire 16S rRNA gene to determine taxonomic distribution in bacterial communities, such as the gut microbiome.

Dr. Jiri Ködding, Director Operations, sees the extension in line with the corporate mission: "At CeGaT, we are committed to helping scientists realize their research projects. Therefore, we provide a wide range of sequencing solutions. With Full-Length 16S Sequencing, we have expanded our portfolio with another high-class sequencing service that adds even more depth and quality to the generated genetic data."

The full-length gene can now be sequenced

The highly conserved 16S rRNA gene is approximately 1.500 bp long and consists of nine hypervariable regions (V1 – V9) that differ between different bacteria. Using PacBio single molecule real-time (SMRT) technology, the entire 16S rRNA gene is sequenced. The technology enables precise long-read sequencing (HiFi reads) using circular consensus sequencing (CCS) mode. As a result, the full-length gene can now be analyzed at CeGaT.

CeGaT’s Full-Length 16S Sequencing is featured by the following:

• fast turnaround times and the highest sequencing accuracy
• analysis of the complete 16S rRNA gene (V1-V9 regions)
• HiFi long reads of approx. 1,500 bp
• simultaneous analysis of multiple samples