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Collaborative Breakthrough in Lung-on-Chip Technology
Collaborative Breakthrough in Lung-on-Chip Technology: AlveoliX, Vitrocell, University of Bern and Helmholtz Institute for Pharmaceutical Research Saarland Drive Advances in Human Respiratory Research AlveoliX, in collaboration with Vitrocell Systems, the University of Bern, and the Helmholtz Institute for Pharmaceutical Research Saarland (HIPS) is proud to announce the publication of a landmark study in Bioengineering & Translational Medicine. The study, titled “A stretchable human lung-on-chip model of alveolar inflammation for evaluating anti-inflammatory drug response”, was made possible through the AIM-4-Doc grant from Eurostars. This study demonstrates how this Lung-on-Chip model provides an advanced platform to study human lung function, offering a more accurate and predictive approach to investigating respiratory diseases and…
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Dry Powder Exposure of Air-Liquid Interface (ALI) Cell Cultures: VITROCELL PowderX System
The VITROCELL PowderX Exposure System is the ideal tool for aerosolisation of small quantities of dry powders and particle deposition on cells cultured in 12- or 24-well sized inserts. It is designed for evaluation of pharmaceutical compounds and toxicological evaluation of particles and fibres. Small powder samples of 1–100 mg are positioned in the quick-lock loading system. Subsequent aerosolisation of the powder takes place under high pressure by a unique jet system. The resulting shear forces facilitate deagglomeration for homogeneous dispersion into the expansion chamber. Final deposition takes place via gravimetric settling. The height of the expansion chamber can be varied in three different steps according to the behaviour and…
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96-well Exposure of Air-Liquid Interface (ALI) Cell Cultures on HTS Plates: New VITROCELL® 96 Continuous Flow Exposure System
The VITROCELL® 96 Continuous Flow Exposure System represents a breakthrough in high-throughput research involving human cell cultures or tissues exposed directly to airborne substances such as gases, complex mixtures and particles, including nanoparticles. This innovative system faithfully replicates the conditions of human physiological exposure at the Air-Liquid Interface (ALI). With the capacity to perform up to 11 dilutions, each with 8 replicates, and dedicating the 12th insert row for clean air control with also 8 replicates, the 96-well plates offer exceptional versatility, allowing for the assessment of multiple endpoints in a single experiment. Key Features The VITROCELL® 96 Exposure System provides a comprehensive 96-well turnkey solution that includes all necessary…
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In vitro lung cell exposure systems by VITROCELL at EUROTOX 2023
VITROCELL displayed the latest lung cell exposure systems for liquid aerosols at EUROTOX 2023: Cloud Alpha 12, Cloud Alpha TRIO, Cloud Alpha MOVE and the lung-on-chip system Cloud Alpha AX12. The Cloud Alpha product group is particularly well suited for exposure of lung cells to scarce and expensive materials, such as new drug candidates or particle samples from the environment. The new advanced dry powder exposure system PowderX catched also a lot of interest. It is specially designed for aerosolisation of smallest quantities of dry powders. The need for only 1-100 mg of test substance makes the system a powerfull tool for lung cell exposure of scarce and very expensive…
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Exposure of Lung Cells to Liquid Aerosols at the Air/Liquid Interface
Good Things Come in Threes: This latest member of the Cloud Alpha family features the delivery of three different aerosol concentrations or three different substances in one experiment. The latter procedure is particularly interesting for users which need to test and compare larger numbers of different substances. The new Cloud Alpha TRIO is capable to expose mammalian cell cultures in 12-and 24-well sized inserts. The Cloud system is suitable for nebulization of solutions and suspensions. Fields of application are screening of inhalable drugs, assessing the toxicity of inhaled substances like chemicals or nanoparticles, and conducting research on viruses. Each aerosol exposure compartment has its dedicated Quartz Crystal Microbalance to assess…
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Dynamic Spirit of Innovation: 2nd MPS World Summit in Berlin
The 2023 World Summit for microphysiological systems (MPS) had a record attendence with over 1,200 participants from all over the world. The increase of attendees from 700 at the 2022 MPS Meeting in New Orleans showed the dynamics in this emerging field. 196 speakers reported on their research using organs-on-chip. The conference started with a very successful Educational Workshop where VITROCELL explained the exposure to the AlveoliX AX12 lung-on-chip using the exposure system Cloud Alpha AlveoliX AX12. Clémentine Richter from the Helmholtz Institute for Pharmaceutical Research Saarland, Saarbrücken, Germany presented her poster on alveolar inflammation illustrating human drug response using the Cloud Alpha AlveoliX AX12 (See Poster) The exhibition gave…
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VITROCELL® Remote Assist Support
VITROCELL in-vitro-Expositionssysteme werden spezifisch nach Kundenwunsch gefertigt. Unsere Kunden aus Forschung & Entwicklung haben meist sehr komplexe Anforderungen an die Anlage. Daher werden Produktschulungen sowie Service & Support von qualifizierten VITROCELL Technikern durchgeführt. Für VITROCELL Anwender auf der ganzen Welt möchten wir stets eine schnelle Reaktion und Problemlösung ermöglichen. Live Assistance mit Augmented Reality Eine enorme Erleichterung bietet hier der Einsatz von Augmented Reality. Bei VITROCELL setzen wir auf die HoloLens 2 von Microsoft. Sie erlaubt unserem Support, in Echtzeit passgenaue Anleitungen zu geben. Das Sichtfeld des Anwenders wird auf den Monitor des Technikers übertragen. Dieser kann so eine Schulung der Anlage aus der Ferne durchführen, oder Möglichkeiten zur…
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VITROCELL® AES for toxicity testing of airborne particles
DOI:10.3791/61210 Hedwig M. Braakhuis1, Ruiwen He1,2, Rob J. Vandebriel1, Eric R. Gremmer1, Edwin Zwart1, Jolanda P. Vermeulen1, Paul Fokkens1, John Boere1, Ilse Gosens1, Flemming R. Cassee1,2 1National Institute for Public Health and the Environment (RIVM) 2Institute for Risk Assessment Sciences (IRAS) This article provides a method for culturing and exposing the human bronchial epithelial cell line Calu-37 at the Air-liquid Interface that mimics realistic, repeated inhalation exposure conditions that can be used for toxicity testing. By applying a continuous airflow using the Automated Exposure System, the cell model can be exposed to a low concentration of particles over a longer time period, reflecting realistic exposure conditions. Characteristics of both the…
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New VITROCELL® Millicell Holder
The revolutionary new holder system enables for the use of standing inserts in all modules of the VITROCELL® 12 Series for 12-well sized inserts. As an additional advantage, the media compartment is sealed towards the aerosol exposure head to avoid contact of the test substance with the cell culture media. VITROCELL® Holder system set: Insert holder with o-ring Adaptor Insert mounting tool Holder removal tool Mounting procedure: Place the adaptor in the exposure module. Then mount the insert in the insert holder using the insert mounting tool. Place the insert holder with the mounted insert into the adaptor. Make sure that no air bubbles are situated under the membrane: add…
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The New VITROCELL® RH/T-Controller
The VITROCELL® RH/T-Controller system was designed to measure temperature and relative humidity in numerous applications at high precision. It can be fitted to humidification systems, aerosol ducts and climatic chambers. In cell exposure, relative humidity is a key factor for cell viability – this is even more important at longer exposure durations. With the new RH/T Controller set VITROCELL® has brought the technology from the Automated Stations to custom configurations. The sensors are availabe in 2 insertion lengths ( 35 mm and 65 mm). They come calibrated with a factory certificate. Re-calibration can be performed using the VITROCELL® Monitor software. VITROCELL® Monitor Software The VITROCELL® RH/T-Controller can be operated as…
